Restricted Research - Award List, Note/Discussion Page

Fiscal Year: 2018

2004  The University of Texas at San Antonio  (75822)

Principal Investigator: Mayer, Kathryn (Principal Investigator)  

Total Amount of Contract, Award, or Gift (Annual before 2011): $ 433,950

Exceeds $250,000 (Is it flagged?): Yes

Start and End Dates: 4/1/18 - 3/31/21

Restricted Research: YES

Academic Discipline: COS PHYSICS & ASTRONOMY  

Department, Center, School, or Institute: Center for Research and Training in the Sciences (CRTS)  

Title of Contract, Award, or Gift: Single-Particle Characterization of Nanoparticle Bioconjugates

Name of Granting or Contracting Agency/Entity: Natl Inst of Health
CFDA Link: HHS
93.859

Program Title: N/A
CFDA Linked: Biomedical Research and Research Training

Note:

Nanoparticles play several roles in biomedical technology, including as imaging contrast agents, heat sources for photothermal therapy, and as drug delivery vehicles. In many of these applications, nanoparticles are functionalized with specific antibodies for targeting purposes. Ideally, antibodies would be distributed equally among the nanoparticles in a preparation and evenly distributed on each nanoparticles surface; however, this is an overly simplistic picture. In reality, there are likely to be variations in the number of antibodies per nanoparticle, their spatial distribution on the surface, and the degree to which their function is preserved. In order to characterize nanoparticle bioconjugates, and to design improved ones, the details of the surface functionalization must be better understood. The following two aims will pave the way towards this goal. (1) The distributions of the number of antibodies per nanoparticle within a nanoparticle preparation will be measured at the single-particle level using microwell array analysis, for both gold and polystyrene nanoparticles. (2) The spatial arrangement of antibodies on the surfaces of individual nanoparticles will be imaged using sub-diffraction-limited fluorescence microscopy. The preparation methods will be optimized to produce nanoparticles with uniform functionalization in terms of the number and spatial distribution of molecules on the surface. Characterizing and optimizing the surface functionalization is important for the development of nanoparticle-based therapies. Improving the uniformity and quality of antibody functionalization will lead to improved targeting. The project will be carried out in the Department of Physics and Astronomy at the University of Texas at San Antonio, which is one of the countrys largest designated Hispanic Serving Institutions. An important part of this SCORE project will be the training of Ph.D. students from groups historically underrepresented in the physical and health sciences, and the elevation of research at this growing institution.

Discussion: No discussion notes

 

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